cd45 pe cy7 bd Search Results


cd45 2 pe cy7  (R&D Systems)
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R&D Systems cd45 2 pe cy7
Cd45 2 Pe Cy7, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd45 antibody, anti-human, pe  (Miltenyi Biotec)
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Miltenyi Biotec cd45 antibody, anti-human, pe
Cd45 Antibody, Anti Human, Pe, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pe cy7  (Biorbyt)
91
Biorbyt pe cy7
Interaction between BMECs and HCC cells enhances tumor growth in the spine. (A) si-CX3CL1 was transfected into BMECs to construct CX3CL1 low BMECs. Transwell assays were used to examine recruitment of BMECs co-cultured with or without HMCC97H cells. The number of the recruited cells was quantified. n=3. Cell recruitment were analyzed using ANOVA test. (B) Cell vitality of BMECs was assessed using CCK-8 assay; n=3. Data were analyzed using ANOVA followed by Tukey's test. (C) Tumor size was measured based on the volume. n=5. Scale bar, 5 mm. Data were analyzed using an unpaired t-test. (D) Samples from 2 groups were examined using immunofluorescence. Magnification, ×20. The result was analyzed using an unpaired t-test. Gray bars represent the CX3CL1 nor BMECs group and black bars represent the CX3CL1 low BMECs group (E) Flow cytometry revealed the percentage and number of F4/80 + CD11b + cells; n=5. (F) Flow cytometry was used for detecting expression of M2 macrophage surface markers, CX3CR1, MRC1 and CD163; n=5. Cells were selected based on higher expression of <t>CD45</t> for macrophages. Subsequently, CD11b and F4/80 expression were used for further characterization of macrophages. FACS (fluorescent-activated cell sorting) data were analyzed using a Kruskal Wallis test followed by Dunn's non-parametric post hoc test. HCC, hepatocellular carcinoma; BMECs, bone marrow endothelial cells; si, small interfering; CCK-8, Cell Counting kit-8; CX3CL1, C-X3-C motif chemokine ligand 1.
Pe Cy7, supplied by Biorbyt, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fivecolor (cd45-percp-cy5.5, cd19-pe-cy7, cd38fitc, cd138-apc, cd56-pe) flow cytometry  (Partec)
90
Partec fivecolor (cd45-percp-cy5.5, cd19-pe-cy7, cd38fitc, cd138-apc, cd56-pe) flow cytometry
Interaction between BMECs and HCC cells enhances tumor growth in the spine. (A) si-CX3CL1 was transfected into BMECs to construct CX3CL1 low BMECs. Transwell assays were used to examine recruitment of BMECs co-cultured with or without HMCC97H cells. The number of the recruited cells was quantified. n=3. Cell recruitment were analyzed using ANOVA test. (B) Cell vitality of BMECs was assessed using CCK-8 assay; n=3. Data were analyzed using ANOVA followed by Tukey's test. (C) Tumor size was measured based on the volume. n=5. Scale bar, 5 mm. Data were analyzed using an unpaired t-test. (D) Samples from 2 groups were examined using immunofluorescence. Magnification, ×20. The result was analyzed using an unpaired t-test. Gray bars represent the CX3CL1 nor BMECs group and black bars represent the CX3CL1 low BMECs group (E) Flow cytometry revealed the percentage and number of F4/80 + CD11b + cells; n=5. (F) Flow cytometry was used for detecting expression of M2 macrophage surface markers, CX3CR1, MRC1 and CD163; n=5. Cells were selected based on higher expression of <t>CD45</t> for macrophages. Subsequently, CD11b and F4/80 expression were used for further characterization of macrophages. FACS (fluorescent-activated cell sorting) data were analyzed using a Kruskal Wallis test followed by Dunn's non-parametric post hoc test. HCC, hepatocellular carcinoma; BMECs, bone marrow endothelial cells; si, small interfering; CCK-8, Cell Counting kit-8; CX3CL1, C-X3-C motif chemokine ligand 1.
Fivecolor (Cd45 Percp Cy5.5, Cd19 Pe Cy7, Cd38fitc, Cd138 Apc, Cd56 Pe) Flow Cytometry, supplied by Partec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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Image Search Results


Interaction between BMECs and HCC cells enhances tumor growth in the spine. (A) si-CX3CL1 was transfected into BMECs to construct CX3CL1 low BMECs. Transwell assays were used to examine recruitment of BMECs co-cultured with or without HMCC97H cells. The number of the recruited cells was quantified. n=3. Cell recruitment were analyzed using ANOVA test. (B) Cell vitality of BMECs was assessed using CCK-8 assay; n=3. Data were analyzed using ANOVA followed by Tukey's test. (C) Tumor size was measured based on the volume. n=5. Scale bar, 5 mm. Data were analyzed using an unpaired t-test. (D) Samples from 2 groups were examined using immunofluorescence. Magnification, ×20. The result was analyzed using an unpaired t-test. Gray bars represent the CX3CL1 nor BMECs group and black bars represent the CX3CL1 low BMECs group (E) Flow cytometry revealed the percentage and number of F4/80 + CD11b + cells; n=5. (F) Flow cytometry was used for detecting expression of M2 macrophage surface markers, CX3CR1, MRC1 and CD163; n=5. Cells were selected based on higher expression of CD45 for macrophages. Subsequently, CD11b and F4/80 expression were used for further characterization of macrophages. FACS (fluorescent-activated cell sorting) data were analyzed using a Kruskal Wallis test followed by Dunn's non-parametric post hoc test. HCC, hepatocellular carcinoma; BMECs, bone marrow endothelial cells; si, small interfering; CCK-8, Cell Counting kit-8; CX3CL1, C-X3-C motif chemokine ligand 1.

Journal: International Journal of Oncology

Article Title: ADAM17-regulated CX3CL1 expression produced by bone marrow endothelial cells promotes spinal metastasis from hepatocellular carcinoma

doi: 10.3892/ijo.2020.5045

Figure Lengend Snippet: Interaction between BMECs and HCC cells enhances tumor growth in the spine. (A) si-CX3CL1 was transfected into BMECs to construct CX3CL1 low BMECs. Transwell assays were used to examine recruitment of BMECs co-cultured with or without HMCC97H cells. The number of the recruited cells was quantified. n=3. Cell recruitment were analyzed using ANOVA test. (B) Cell vitality of BMECs was assessed using CCK-8 assay; n=3. Data were analyzed using ANOVA followed by Tukey's test. (C) Tumor size was measured based on the volume. n=5. Scale bar, 5 mm. Data were analyzed using an unpaired t-test. (D) Samples from 2 groups were examined using immunofluorescence. Magnification, ×20. The result was analyzed using an unpaired t-test. Gray bars represent the CX3CL1 nor BMECs group and black bars represent the CX3CL1 low BMECs group (E) Flow cytometry revealed the percentage and number of F4/80 + CD11b + cells; n=5. (F) Flow cytometry was used for detecting expression of M2 macrophage surface markers, CX3CR1, MRC1 and CD163; n=5. Cells were selected based on higher expression of CD45 for macrophages. Subsequently, CD11b and F4/80 expression were used for further characterization of macrophages. FACS (fluorescent-activated cell sorting) data were analyzed using a Kruskal Wallis test followed by Dunn's non-parametric post hoc test. HCC, hepatocellular carcinoma; BMECs, bone marrow endothelial cells; si, small interfering; CCK-8, Cell Counting kit-8; CX3CL1, C-X3-C motif chemokine ligand 1.

Article Snippet: To stain for the cell surface markers, cells were incubated with following fluorescence-labeled monoclonal antibodies for 30 min on ice respectively: PE-conjugated anti-CD11b (cat. no. 333142; BD Biosciences; 1:300), FITC-conjugated anti-F4/80 (cat. no. orb223773; Biorbyt; 1:300), PE-Cy7-conjugated-CD45 (cat. no. 147703; BioLegend; 1:300), APC-conjugated CX3CR1 (cat. no. 341609; BioLegend; 1:300), PE-Cy3-conjugated MRC1 (cat. no. sc-376232; Santa Cruz Biotechnology, Inc.; 1:300) and PE-Cy5-CD163 (cat. no. sc-33715; Santa Cruz Biotechnology, Inc.; 1:300) antibodies.

Techniques: Transfection, Construct, Cell Culture, CCK-8 Assay, Immunofluorescence, Flow Cytometry, Expressing, FACS, Cell Counting